منابع مشابه
Structural organization of the yeast vacuolar H+-ATPase The yeast vacuolar proton-translocating ATPase
The yeast vacuolar proton-translocating ATPase (VATPase) is structurally very similar to the V-ATPases of other fungi, plants and animals (for reviews, see Stevens and Forgac, 1997; Forgac, 1999; Nelson and Harvey, 1999). In all these systems, the V-ATPase is composed of a complex of peripheral membrane proteins containing the ATP binding sites attached to a complex of integral membrane protein...
متن کاملTopography of a vacuolar-type H+-translocating ATPase: chromaffin-granule membrane ATPase I.
Proteins exposed on the cytoplasmic face of isolated chromaffin granules were labelled by lactoperoxidase-catalysed radioiodination and by non-enzymic biotinylation. Granule membranes were then prepared, and the H+-translocating ATPase isolated by fractionation with Triton X-114. The labelling of individual ATPase subunits was assessed by polyacrylamide-gel electrophoresis, followed by autoradi...
متن کاملPurification and reconstitution of the proton-translocating ATPase of Golgi-enriched membranes.
Kidney cortex microsomes enriched in Golgi markers and probably also containing endosomes were isolated by cell fractionation and found to contain a proton-translocating ATPase that was inhibited by N-ethylmaleimide (NEM). This NEM-sensitive ATPase was solubilized with n-octyl glucoside and purified using anion-exchange sievorptive chromatography on sequential DEAE-Sephadex and QAE-Sephadex col...
متن کاملImportant role for the V-type H(+)-ATPase and the Golgi apparatus in the recycling of PTH/PTHrP receptor.
Our previous studies demonstrated that a green fluorescent protein-tagged parathyroid hormone (PTH)/PTH-related peptide (PTHrP) receptor stably expressed in LLCPK-1 cells undergoes agonist-dependent internalization into clathrin-coated pits. The subcellular localization of the internalized PTH/PTHrP receptor is not known. In the present study, we explored the intracellular pathways of the inter...
متن کاملStudies on T H E Golgi Apparatus by Electron Microscopy
Observations made with the aid of the light microscope have led some workers to believe that the classical osmium and silver impregnation techniques for the Golgi apparatus do not reveal a single homologous inclusion but a variety of dissimilar objects. I t has been variously suggested that the osmium or silver deposits on neutral-red or lipoidal bodies (41-44, 31, 26, 2-7, 53), mitochondria (5...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1989
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)51486-1